In this study, the antioxidant activity of 12 Catalpa bungei clones extracted from He'nan and Gansu was evaluated in order to find the most effective ones with higher antioxidant activity as well as total phenol content. The results showed that CA6 from Gansu Province was the most effective one in reducing ability (41.61%), DPPH radical scavenging activity (the reciprocal of IC50), hydroxyl radical scavenging activity (the reciprocal of IC50) and the highest total phenol content (31.73 mg·g-1) among the 12 clones. The reducing ability and total phenol content determined in 6 clones in Gansu were significantly higher than that in He'nan Province. In addition to CA3, the hydroxyl radical scavenging activity and DPPH radical scavenging activity of the other 5 clones in Gansu were significantly higher than in that He'nan Province. Moreover, the antioxidant activity of ethyl acetate fraction in CA6 leaves was the highest, followed by n-butanol, petroleum ether, and the water fractions was the lowest.